Journal: Biochimica et biophysica acta

Article Title: Platinum-induced kidney damage: Unraveling the DNA damage response (DDR) of renal tubular epithelial and glomerular endothelial cells following platinum injury.

doi: 10.1016/j.bbamcr.2014.12.033

Figure Lengend Snippet: Fig. 1. Tubular (NRK-52E) and glomerular (RGE) cells differ in their sensitivity to platinum compounds. A, B: Logarithmically growing normal rat tubular epithelial cells (NRK-52E) (A) and glomerular endothelial cells (RGE) (B) were incubated with different concentrations of the platinum compounds for 72 h. Cell viability was measured using the MTT assay as described in methods. Results are expressed as viable cells in percent of the untreated control, which was set to 100%. Data shown are the mean ± SD from 3 to 5 independent experiments each performed in quadruplicate. *: significance of CisPt vs. OxaliPt; #: significance of CisPt vs. CarboPt; +: significance of OxaliPt vs. CarboPt. ***, ###, +++p ≤0.001; ++p ≤0.01. C: NRK-52E and RGE cells were treated with the corresponding IC80 of the different platinum compounds. Cell growth was measured by recording alterations in electrical impedance in real-time as described in methods. Shown are data from one representative experiment. Con, untreated control. D: Logarithmically growing rat tubular epithelial (NRK-52E) and glomerular endothelial (RGE) cells were incubated with the IC50 of the platinum compounds for 72 h. The number of viable cells was determined by Trypan Blue exclusion assay as described in methods. The number of viable (i.e. Trypan Blue negative) cells at the time of drug addition (Con2) was set to 100%. Data shown are the mean ± SD from one representative experiment performed in triplicate. Con1, untreated control after incubation period of 72 h; Con2, untreated control at the time of platinum treatment; CisPt, OxaliPt, CarboPt, percent viable cells 72 h after platinum treatment.

Article Snippet: Rat renal proximal tubular epithelial (NRK-52E) and rat glomerular endothelial (RGE) cells originate from the German Collection of Microorgansisms and Cell Culture (DSMZ) (Braunschweig, Germany).

Techniques: Incubation, MTT Assay, Control, Trypan Blue Exclusion Assay